Device

Part:BBa_K2874001:Design

Designed by: Rishabh Jain   Group: iGEM18_IIT_Kanpur   (2018-10-09)


JA105->PelB-sfGFP-His tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 113
    Illegal NgoMIV site found at 837
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have included Nru1 site before sfGFP sequence. This might be used for cloning purpose of any protein of interest by an iGEM team. Though verification that cloning took place has to be designed separately for different proteins.


Source

This sequence is a composite part of existing biobricks.

Component: Bba_J23105 (JA105)


References